Publication Details:
Journal or Publication Title:
Armenian Journal of Physics=Ֆիզիկայի հայկական հանդես
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Title:
Nondestructive Label-Free Mapping of DNA Bioassay Using a Near-Field Scanning Microwave Microscope
Creator:
Babajanyan, A. ; B. Friedman ; K. Lee
Corporate Creators:
Yerevan State University, Department of Radiophysics ; Department of Physics, Sam Houston State University, Huntsville, Texas 77341, USA ; Department of Physics and Basic Science Institute for Cell Damage Control Sogang University, Seoul, Korea
Subject:
Physics ; Electronics; radiowave and microwave technology; direct energy conversion and storage ; Cross-Disciplinary
Coverage:
Abstract:
Near-field scanning microwave microscope (NSMM) is used to readout and visualize homemade 10mer oligonucleotides microarrays of DNA. NSMM instrumentation does not require labeling of target sequences with fluorophores or other tagging groups. Sensitive examination of DNA coverage and high resolution mapping of DNA spots in the microarray were observed by measuring thechange of microwave reflection coefficient (S11) at about 4 GHz operating frequency. The linear dynamicrange for the 10mer nucleotide is over at least 3 orders of magnitude from 0.001pM to 1pM with the detection limit estimated to be approximately 1fM, i.e. 21010 stands/m2.